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PacBio was founded in 2004 to develop sequencing methods based on work done in the labs of biophysicist Watt Webb and engineer Harold Craighead, both at Cornell University. The previous year, the two had collaborated to create zero-mode waveguides (ZMWs): small containers just big enough to hold a single DNA polymerase and containing tiny holes at the bottom through which light could be detected. They were able to fix a DNA polymerase to the bottom of a ZMW and detect the incorporation of individual fluorescent “C” nucleotides through the holes, which fed into a microscope capable of detecting light emissions.

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Tusk Says

By default, ffmpeg compresses the images quite a bit. It was enough that I could notice a quality difference when I put a paused frame of the video side-by-side with an extracted image. We can force ffmpeg to improve the quality with q:v (number). A smaller number here produces a higher quality image at the expense of file size and processing time. I’ve settled on a value of 3, but feel free to play around with this to get the quality or file sizes you want.